Thomander L, Aldskogius H, Grant G
Acta Otolaryngol. 1982 May-Jun;93(5-6):397-405. doi: 10.3109/00016488209130897.
The intra-axonal transport of horseradish peroxidase (HRP) was used to label fibres in the intratemporal facial nerve portion (ITFN) in the rat and cat. HRP was applied to the proximal cut end of facial nerve branches innervating different facial muscles. Following appropriate survival periods, the animals were fixed by perfusion. The ITFN was processed histochemically for demonstration of intra-axonal HRP, using tetramethylbenzidine as substrate. The distribution of labelled fibres was analyzed in serial longitudinal sections and in photographic transverse-like reconstructions from three selected levels. HRP-labelled fibres from all examined motor branches were found to be diffusely distributed almost throughout the entire ITFN, leaving only a narrow zone free of label. By applying HRP to the chorda tympani nerve, it was shown that this zoe harbours fibres of this nerve. The findings support the conclusion that the motor fibres to the facial muscles are diffusely organized in the ITFN.
利用辣根过氧化物酶（HRP）的轴突内运输来标记大鼠和猫颞内面神经部分（ITFN）中的纤维。将HRP应用于支配不同面部肌肉的面神经分支的近端切断端。在适当的存活期后，通过灌注固定动物。使用四甲基联苯胺作为底物，对ITFN进行组织化学处理以显示轴突内HRP。在连续的纵向切片以及从三个选定水平的类似摄影横向重建中分析标记纤维的分布。发现来自所有检查的运动分支的HRP标记纤维几乎分散分布在整个ITFN中，仅留下一个没有标记的狭窄区域。通过将HRP应用于鼓索神经，表明该区域含有该神经的纤维。这些发现支持以下结论：支配面部肌肉的运动纤维在ITFN中呈分散排列。
