Persinger M A
Behav Neural Biol. 1983 Nov;39(2):299-306. doi: 10.1016/s0163-1047(83)90995-0.
Quantitative measurements were made of brain mast cell (MC) degranulation in juvenile albino rats. Neither acute nor chronic intraperitoneal injections of Compound 48/80 (C 48/80) evoked any clear degranulation. Fifteen to thirty minutes after the injection of either C 48/80 or physiological saline into the right anterior thalamus, frank degranulation in the leptomeninges and some degranulation in the parenchyma were evident. The injected sides contained about twice as much degranulated cells as the noninjected sides. In a second experiment, animals were killed 10, 100, 1000, and 10,000 min after a single 10-microliters injection of C 48/80 into the anterior thalamus. Although about 40% of the leptomeningeal and 20% of the parenchymal MCs were degranulated within 10 min, more than 50% of the MCs in both areas were degranulated within 100 min after the injection. More extensive degranulation was evident at both times within the parenchyma of the injected sides. Degranulated MCs were not obvious after this period although nuclei surrounded by sparse granules (in the parenchyma) or by fused, globular metachromatic material (in the leptomeninges or their ventricular processes) were still discernable 7 days later. The implications of brain mast cell degranulation for psychoneuroimmunology are considered.
对幼年白化大鼠脑肥大细胞（MC）脱颗粒进行了定量测量。腹腔内急性或慢性注射化合物48/80（C 48/80）均未引起明显的脱颗粒。将C 48/80或生理盐水注入右前丘脑后15至30分钟，软脑膜出现明显的脱颗粒，实质内也有一些脱颗粒。注射侧的脱颗粒细胞数量约为未注射侧的两倍。在第二个实验中，在将10微升C 48/80单次注入前丘脑后10、100、1000和10000分钟处死动物。虽然在10分钟内约40%的软脑膜MC和20%的实质MC发生了脱颗粒，但注射后100分钟内两个区域超过50%的MC发生了脱颗粒。在注射侧的实质内，这两个时间点的脱颗粒更为广泛。在此之后，脱颗粒的MC不明显，尽管7天后仍可辨认出被稀疏颗粒包围的细胞核（在实质内）或被融合的球状异染性物质包围的细胞核（在软脑膜或其脑室突起内）。本文考虑了脑肥大细胞脱颗粒对精神神经免疫学的影响。
