Mayr B, Schweizer D, Schleger W
Can J Genet Cytol. 1983 Dec;25(6):616-21. doi: 10.1139/g83-092.
The application of the counterstain-contrasted fluorescent banding technique to canine chromosomes provided an improved capability to highlight specific heterochromatic regions and to produce well defined banding patterns both on mitotic and meiotic chromosomes. Triple staining with chromomycin A3 - distamycin A - DAPI revealed the occurrence of DA - DAPI positive heterochromatin in chromosomes 33, 36, 37, and 38. Pachytene nuclei present more favourable material for the detection of very small amounts of DA - DAPI material than mitotic division stages. Counterstain-enhanced chromomycin R-banding greatly facilitated chromosome identification. A standard R-band karyotype of Canis familiaris is proposed and described in some detail. DAPI - actinomycin D staining produced a QFH-type banding pattern and enhanced differentiation of some polymorphic regions.
将抗染色剂对比荧光显带技术应用于犬染色体，提高了突出特定异染色质区域以及在有丝分裂和减数分裂染色体上产生清晰显带模式的能力。用放线菌素A3 - 偏端霉素A - 4',6-二脒基-2-苯基吲哚（DAPI）进行三重染色，揭示了33、36、37和38号染色体上存在偏端霉素A - DAPI阳性异染色质。与有丝分裂期相比，粗线期核为检测极少量的偏端霉素A - DAPI物质提供了更有利的材料。抗染色剂增强的放线菌素R显带极大地促进了染色体识别。提出并详细描述了家犬的标准R带核型。DAPI - 放线菌素D染色产生了QFH型显带模式，并增强了一些多态性区域的分化。
