Fujikawa-Yamamoto K
Cell Struct Funct. 1983 Sep;8(3):303-8. doi: 10.1247/csf.8.303.
The cell cycle of HeLa S3 cells synchronized by hydroxyurea was investigated by flow cytometry. Metachromatic fluorochrome acridine orange was used to strain the DNA and RNA of the cells differentially. Periodic changes in the cellular DNA and RNA contents were observed through five cell cycles. The G1 and S phases of synchronized HeLa S3 cells that contained large amounts of RNA became shorter than those of cells that contained smaller amounts of RNA.
通过流式细胞术研究了经羟基脲同步化的HeLa S3细胞的细胞周期。使用异染荧光染料吖啶橙对细胞的DNA和RNA进行差异染色。在五个细胞周期中观察到细胞DNA和RNA含量的周期性变化。含有大量RNA的同步化HeLa S3细胞的G1期和S期比含有较少RNA的细胞的相应时期更短。
