Fujikawa-Yamamoto K
J Cell Physiol. 1982 Jul;112(1):60-6. doi: 10.1002/jcp.1041120110.
The cell cycle of V79 Chinese hamster lung cells synchronized by hydroxyurea was investigated by flow cytometry. The metachromatic fluorochrome acridine orange was used to differentially stain DNA and RNA of V79 cells. Green and red fluorescence from individual cells, representing cellular DNA and RNA, respectively, was measured by flow cytometry. Periodic changes of cellular DNA and RNA contents were observed over nine cell cycles. The duration of G1, S, and G2 + M phases of synchronized V79 cells whose RNA content was close to that of the cells in balanced growth was 3, 4.5, and 1.5 hours, respectively. The duration of G1 and S phases of cells containing RNA above a certain threshold was inversely proportional to the RNA content. The RNA content of cells containing RNA above the normal level regressed to normal after a few generations. Coefficients of variation for RNA content were significantly larger than those for DNA. An explanation for the decay of synchrony in a synchronized cell population is proposed.
通过流式细胞术研究了经羟基脲同步化的V79中国仓鼠肺细胞的细胞周期。使用异染荧光染料吖啶橙对V79细胞的DNA和RNA进行差别染色。通过流式细胞术测量单个细胞分别代表细胞DNA和RNA的绿色和红色荧光。在九个细胞周期中观察到细胞DNA和RNA含量的周期性变化。RNA含量接近平衡生长细胞的同步化V79细胞的G1、S和G2+M期持续时间分别为3、4.5和1.5小时。RNA含量高于一定阈值的细胞的G1和S期持续时间与RNA含量成反比。RNA含量高于正常水平的细胞经过几代后RNA含量回归正常。RNA含量的变异系数显著大于DNA的变异系数。提出了同步化细胞群体中同步性衰退的一种解释。
