Kraus H
Fortschr Med. 1983 Dec 15;101(47-48):2189-94.
In pap-stained cervical smears the cells were identified, destained and afterwards stained according to the Feulgen procedure. The measurements of the nuclei were performed on a scanning microscope photometer and 4 features were extracted for cell classification: total DNA-amount, nuclear area, degree of condensation of chromatin and granularity of chromatin. The comparison and separation of the cell groups was performed at the Computing Center of the Univ. of Ulm by 2 programs, a discriminant analysis and a specially adapted histogram. The distinction of normal cells and dyscaryoses was possible to a high degree by the features total DNA-amount and nuclear area; the 2 parameters describing the chromatin structure proved to be useless in these cases. Nuclear structure parameters were helpful in distinguishing certain types of normal cells.
在巴氏染色的宫颈涂片上，细胞被识别、脱色，然后按照福尔根染色法进行染色。细胞核的测量在扫描显微镜光度计上进行，并提取4个特征用于细胞分类：总DNA含量、核面积、染色质凝聚程度和染色质颗粒度。细胞组的比较和分离在乌尔姆大学计算中心通过两个程序进行，即判别分析和一个特别适配的直方图。通过总DNA含量和核面积这两个特征，能够高度区分正常细胞和发育异常细胞；在这些情况下，描述染色质结构的两个参数被证明是无用的。核结构参数有助于区分某些类型的正常细胞。
