Schwarz J, Kraus H, Raffler H
Microsc Acta. 1979 Jan;81(3):201-8.
An earlier published [8] image analysis system for cytology is tested on gynecological material. At first the question for the staining method which is optimal for scanning-photometrical measurements is investigated. In addition the wavelength of the light which reproduces best the nuclear structure must be found. Feulgen-stained preparations scanned at 570nm allow a more secure sorting of superficial cells against intermediary cells than those in the classical Papanicolaou-stain at white light. Discoloring of Papanicolaou-preparations and Feulgen staining afterwards was accomplished without technical problems and there were not detectable any qualitative differences with directly Feulgen-stained preparations by means of image analysis. With this method it is possible to recognize structural changes in nuclei of vaginal epithelium during cell differentation.
一个早期发表的[8]用于细胞学的图像分析系统在妇科材料上进行了测试。首先，研究了哪种染色方法最适合扫描光度测量。此外，必须找到能最佳再现核结构的光的波长。与在白光下进行经典巴氏染色的标本相比，在570nm波长下扫描的福尔根染色标本能更可靠地将表层细胞与中间细胞区分开来。巴氏染色标本脱色后再进行福尔根染色没有技术问题，并且通过图像分析未检测到与直接福尔根染色标本有任何质量差异。用这种方法可以识别阴道上皮细胞核在细胞分化过程中的结构变化。
