Ohkawa K, Tsukada Y, Hibi N, Hirai H
Int J Cancer. 1984 Apr 15;33(4):497-502. doi: 10.1002/ijc.2910330413.
The inhibitory effects of horse antiserum against rat alpha-fetoprotein (AFP) on the uptake of 2-deoxy-D-glucose (2dG) by the AFP-producing rat ascites hepatoma AH66 cells was studied. AH66 cells cultured in medium containing 20% heat-inactivated antiserum had a 1.5-fold lower rate of sugar uptake than did AH66 cells which were cultured in medium containing 20% heat inactivated normal horse serum. The inhibition of 2dG uptake by antiserum was dependent on both the concentration and the exposure time of antiserum. Preincubation of AH66 tumor cells for 2 and 6 h with antiserum prior to the measurement of 2dG uptake resulted in a 70.1% and 58.2% decrease in 2dG uptake compared to control cells. Antiserum did not inhibit the rate of phosphorylation of 2dG by tumor cells. Kinetic constants for the uptake of 2dG in both AH66 cells treated with antiserum to AFP and in control cells were calculated from Lineweaver-Burk plots. The Km remained constant at approximately 1.2 mM, but the Vmax was twice as small for the cells treated with antiserum as for the control cells (571 vs 923 nanomoles/2 X 10(5) cells/min). These studies suggest that the inhibition of 2dG uptake by treatment with antiserum was the result of a decrease in the number of transport sites, or a decrease in the amount of carrier protein for the sugar which was present on the surface of the plasma membrane of the AH66 cells.
研究了马抗大鼠甲胎蛋白（AFP）抗血清对产生AFP的大鼠腹水肝癌AH66细胞摄取2-脱氧-D-葡萄糖（2dG）的抑制作用。在含有20%热灭活抗血清的培养基中培养的AH66细胞，其糖摄取率比在含有20%热灭活正常马血清的培养基中培养的AH66细胞低1.5倍。抗血清对2dG摄取的抑制作用取决于抗血清的浓度和作用时间。在测量2dG摄取之前，将AH66肿瘤细胞与抗血清预孵育2小时和6小时，与对照细胞相比，2dG摄取分别减少了70.1%和58.2%。抗血清不抑制肿瘤细胞对2dG的磷酸化速率。根据Lineweaver-Burk图计算了用AFP抗血清处理的AH66细胞和对照细胞中2dG摄取的动力学常数。Km保持在约1.2 mM不变，但抗血清处理的细胞的Vmax仅为对照细胞的一半（571对923纳摩尔/2×10⁵细胞/分钟）。这些研究表明，用抗血清处理抑制2dG摄取是转运位点数量减少或AH66细胞质膜表面存在的糖载体蛋白量减少的结果。
