抗猪透明带抗原单克隆抗体及其对受精的抑制作用。

Monoclonal antibodies to porcine zona pellucida antigens and their inhibitory effects on fertilization.

作者信息

Isojima S, Koyama K, Hasegawa A, Tsunoda Y, Hanada A

出版信息

J Reprod Immunol. 1984 Feb;6(2):77-87. doi: 10.1016/0165-0378(84)90002-0.

PMID:6200596

Abstract

Five hybridomas which produce monoclonal antibodies (Mabs) to porcine zona pellucida (ZP) were established. The immunoglobulin classes were IgG2a from hybridoma B11C8 and IgM from the other four. Using immunofluorescent staining, the Mab from B11C8 stained only porcine oocytes, the Mab from G10G5 stained oocytes of pigs and hamsters but Mabs from C6H1, D3H4, G10F9 stained oocytes of pigs, humans, hamsters, rats and mice. Mabs from B11C8 and G10F9 strongly blocked boar sperm binding to porcine ZP but other Mabs produced only slight blocking. However, no Mabs could block sperm penetration during in vitro fertilization (IVF) of hamster oocytes. When a goat antibody (IgG) to mouse serum gamma-globulin was applied after each Mab as a second antibody, only Mab from G10G5 impaired IVF of hamster oocytes.

摘要

建立了五株产生抗猪透明带（ZP）单克隆抗体（Mab）的杂交瘤。免疫球蛋白类别为来自杂交瘤B11C8的IgG2a和来自其他四株的IgM。使用免疫荧光染色，来自B11C8的Mab仅对猪卵母细胞染色，来自G10G5的Mab对猪和仓鼠的卵母细胞染色，但来自C6H1、D3H4、G10F9的Mab对猪、人、仓鼠、大鼠和小鼠的卵母细胞染色。来自B11C8和G10F9的Mab强烈阻断公猪精子与猪ZP的结合，但其他Mab仅产生轻微阻断。然而，在仓鼠卵母细胞的体外受精（IVF）过程中，没有Mab能够阻断精子穿透。当在每种Mab之后应用抗小鼠血清γ-球蛋白的山羊抗体（IgG）作为第二抗体时，只有来自G10G5的Mab损害了仓鼠卵母细胞的IVF。