Mori T, Kamada M, Yamano S, Kinoshita T, Kano K, Mori T
J Reprod Immunol. 1985 Aug;8(1):1-11. doi: 10.1016/0165-0378(85)90073-7.
Two hybridoma cell lines producing murine monoclonal antibodies to antigens common to the zona pellucida (ZP) of pigs and humans were obtained by immunization of mice with solubilized porcine zona antigen. Indirect immunofluorescence tests showed that both these monoclonal antibodies stained the entire layer of porcine ZP but stained different regions of human ZP, one staining the entire layer and the other only the outer surface. At high concentrations, these two monoclonal antibodies directed against antigens common to porcine and human ZP prevented sperm binding and penetration into human ZP in vitro, whereas a monoclonal antibody directed against an antigen restricted to porcine ZP did not have these inhibitory effects. It is concluded that human and porcine ZP share at least two antigens with different locations in the ZP, and that these influence or are essential for interaction of human sperm with the ZP. These results provide a rationale for using porcine ZP clinically as a vaccine for human immunocontraception.
用溶解的猪透明带抗原免疫小鼠，获得了两株产生针对猪和人透明带（ZP）共同抗原的鼠单克隆抗体的杂交瘤细胞系。间接免疫荧光试验表明，这两种单克隆抗体均能染色猪ZP的整个层，但对人ZP的不同区域进行染色，一种染色整个层，另一种仅染色外表面。在高浓度下，这两种针对猪和人ZP共同抗原的单克隆抗体在体外可阻止精子与人ZP的结合和穿透，而针对仅存在于猪ZP上的抗原的单克隆抗体则没有这些抑制作用。得出的结论是，人和猪的ZP至少共享两种在ZP中位置不同的抗原，并且这些抗原影响或对人精子与ZP的相互作用至关重要。这些结果为临床上将猪ZP用作人类免疫避孕疫苗提供了理论依据。
