Kudryk B, Rohoza A, Ahadi M, Chin J, Wiebe M E
Mol Immunol. 1984 Jan;21(1):89-94. doi: 10.1016/0161-5890(84)90093-2.
A monoclonal antibody (MAb/T2G1s) was prepared by fusion using spleen cells from mice immunized with the NH2-terminal CNBr fragment of human fibrin II, the so-called (T)N-DSK [(A alpha 17-51, B beta 15-118, gamma 1-78)2]. In competition experiments, this antibody reacted with (T)N-DSK as well as peptide B beta 15-42 which can be obtained from (T)N-DSK by digestion with plasmin. Little or no reaction was observed with intact fibrinogen, the NH2-terminal CNBr fragments from fibrinogen (N-DSK) or fibrin I [(B)N-DSK], respectively, as well as peptide B beta 1-42. These results suggest that MAb/T2G1s is directed to an epitope on the B beta chain in fibrin II but not in fibrinogen or fibrin I. As such, MAb/T2G1s differs completely from another antibody (MAb/1-8C6)--also specific for the NH2-terminal region of the B beta chain--which was recently described [Kudryk et al. (1983) Molec. Immun. 20, 1191-1200].
用人类纤维蛋白II的氨基末端溴化氰片段（即所谓的(T)N-DSK [(Aα17 - 51, Bβ15 - 118, γ1 - 78)2]）免疫小鼠后获取的脾细胞进行融合，制备出一种单克隆抗体（MAb/T2G1s）。在竞争实验中，该抗体与(T)N-DSK以及肽Bβ15 - 42发生反应，肽Bβ15 - 42可通过用纤溶酶消化(T)N-DSK得到。分别与完整的纤维蛋白原、纤维蛋白原的氨基末端溴化氰片段（N-DSK）或纤维蛋白I [(B)N-DSK]以及肽Bβ1 - 42几乎没有反应。这些结果表明，MAb/T2G1s针对的是纤维蛋白II中Bβ链上的一个表位，而非纤维蛋白原或纤维蛋白I中的表位。因此，MAb/T2G1s与另一种也对Bβ链氨基末端区域具有特异性的抗体（MAb/1 - 8C6）完全不同，后者是最近报道的[库德里克等人（1983年），《分子免疫学》20卷，1191 - 1200页]。
