Sakamoto H, Kimura N, Nagawa F, Shimura Y
Nucleic Acids Res. 1983 Dec 10;11(23):8237-51. doi: 10.1093/nar/11.23.8237.
The gene coding for the RNA component of RNase P was cloned from a temperature-sensitive mutant of Escherichia coli defective in RNase P activity (ts709) and its parental wild-type strain (4273), and the complete nucleotide sequences of the gene and its flanking regions were determined. The 5'- and 3'-terminal sequences of the RNA component were determined and mapped on the DNA sequence. The mutant gene has GC-to-AT substitutions at positions corresponding to 89 and 365 nucleotides downstream from the 5' terminus of the RNA sequence. Comparing to the wild-type RNA, the mutant RNA is less stable and rapidly degraded in vivo and in vitro.
从核糖核酸酶P（RNase P）活性有缺陷的大肠杆菌温度敏感突变体（ts709）及其亲本野生型菌株（4273）中克隆了编码RNase P的RNA组分的基因，并测定了该基因及其侧翼区域的完整核苷酸序列。确定了RNA组分的5'和3'末端序列，并将其定位在DNA序列上。突变基因在与RNA序列5'末端下游89和365个核苷酸相对应的位置发生了从GC到AT的替换。与野生型RNA相比，突变型RNA在体内和体外都更不稳定且降解迅速。
