Motamedi H, Lee Y, Schmidt F J
Proc Natl Acad Sci U S A. 1984 Jul;81(13):3959-63. doi: 10.1073/pnas.81.13.3959.
The nucleotide sequence of a cloned gene for the RNA component of Escherichia coli ribonuclease P, M1 RNA, is presented. The sequence determined extends 320 nucleotides upstream of the 377-base-pair (bp) structural gene and includes three sequences homologous to the consensus E. coli promoter sequence. Two nucleotides found in the M1 RNA structural gene sequence were not found in a previously determined gene sequence of another M1 RNA clone [Reed, R. E., Baer, M. F., Guerrier-Takeda, C., Donis-Keller, H. & Altman, S. (1982) Cell 30, 627-636]. In vitro transcription of supercoiled plasmid DNA containing the M1 RNA gene resulted in a major transcript arising from the strong promoter nearest to the mature M1 RNA. RNAs encoded by the M1 RNA clone in vivo were examined by S1 nuclease mapping. The results indicated that in vivo transcripts originate from all three promoters preceding the M1 RNA gene. These transcripts are apparently processed in a multistep pathway to generate the 5' end of mature M1 RNA.
本文给出了大肠杆菌核糖核酸酶P的RNA组分M1 RNA的克隆基因的核苷酸序列。所测定的序列在377个碱基对（bp）的结构基因上游延伸了320个核苷酸，并包含三个与大肠杆菌启动子共有序列同源的序列。在M1 RNA结构基因序列中发现的两个核苷酸，在另一个M1 RNA克隆先前测定的基因序列中未被发现[里德，R.E.，贝尔，M.F.，盖里耶-武田，C.，多尼斯-凯勒，H.和阿尔特曼，S.（1982年）《细胞》30卷，627 - 636页]。含有M1 RNA基因的超螺旋质粒DNA的体外转录产生了一个主要转录本，该转录本源自最靠近成熟M1 RNA的强启动子。通过S1核酸酶图谱分析研究了体内由M1 RNA克隆编码的RNA。结果表明，体内转录本源自M1 RNA基因之前的所有三个启动子。这些转录本显然通过多步途径进行加工，以产生成熟M1 RNA的5'端。
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