Donis-Keller H
Nucleic Acids Res. 1980 Jul 25;8(14):3133-42. doi: 10.1093/nar/8.14.3133.
Physarum polycephalum, an acellular slime mold, produces an endoribonuclease activity (Phy M) useful in direct RNA sequence determination. Under conditions previously described for direct enzymatic RNA sequencing (7M urea, 50 degrees C, pH 5.0)1 Phy M cleaves almost exclusively and uniformly at Up-N and Ap-N. In the absence of urea Up-N, Ap-N, and Gp-N are attacked in a sequence specific way identical to that found with RNase Phy I2 digests performed under the same reaction conditions.
多头绒泡菌，一种非细胞黏菌，能产生一种在直接RNA序列测定中有用的核糖核酸内切酶活性（Phy M）。在先前描述的直接酶促RNA测序条件下（7M尿素，50℃，pH 5.0），Phy M几乎只在Up-N和Ap-N处均匀切割。在没有尿素的情况下，Up-N、Ap-N和Gp-N会以与在相同反应条件下进行的RNase Phy I消化所发现的相同的序列特异性方式受到攻击。
