Krol A, Ebel J P, Rinke J, Luhrmann R
Nucleic Acids Res. 1983 Dec 20;11(24):8583-94. doi: 10.1093/nar/11.24.8583.
U1, U2 and U5 RNAs were isolated from pea nuclei with antibody specific for 2,2,7-trimethylguanosine. The nucleotide sequence of the 3'-terminal halves of pea U1 and U2 snRNAs and the complete sequence of five out of the six U5 RNA variants isolated is given. The high number of U5 variants suggest they are encoded by a multigene family containing at least six different genes. Similar secondary structures could be derived for all of the pea U5 RNAs and although the degree of sequence conservation between plant and vertebrate U5 RNAs is as low as 35%, nearly identical secondary structures can be proposed for both RNA groups. All the snRNA species U1, U2 and U5 from pea share a structural domain, the so-called domain A, which is also common to all animal snRNA U1, U2, U4 and U5. Furthermore, a block of 22 consecutive nucleotides is conserved among pea and vertebrate U5 RNAs, from which 11 nucleotides constitute a hairpin-loop with a high number of posttranscriptional modifications. We propose that conservation of this hairpin-loop, together with domain A, is of prime importance for the functioning of U5 RNAs in plant and animal cells.
使用针对2,2,7 - 三甲基鸟苷的特异性抗体从豌豆细胞核中分离出U1、U2和U5 RNA。给出了豌豆U1和U2 snRNA 3'端后半部分的核苷酸序列以及所分离出的六个U5 RNA变体中五个的完整序列。大量的U5变体表明它们由一个至少包含六个不同基因的多基因家族编码。所有豌豆U5 RNA都可以推导得出相似的二级结构，尽管植物和脊椎动物U5 RNA之间的序列保守程度低至35%，但可以为这两类RNA提出几乎相同的二级结构。豌豆的所有snRNA种类U1、U2和U5都共享一个结构域，即所谓的结构域A，这在所有动物snRNA U1、U2、U4和U5中也很常见。此外，豌豆和脊椎动物U5 RNA之间有22个连续核苷酸的区域是保守的，其中11个核苷酸构成一个有大量转录后修饰的发夹环。我们提出，这个发夹环与结构域A的保守性对于U5 RNA在植物和动物细胞中的功能至关重要。
