Krasnova N I, Nagieva F G, Bektemirov T A, Dorofeev V M, Gordienko N M
Vopr Virusol. 1984 Jan-Feb;29(1):51-5.
Reproduction of the vaccine L-3 strains of mumps virus was studied in cultures of continuous Vero cells and in primary cultures of Japanese quail embryos (JQE) growing on DEAE-Sephadex A-50 microcarriers. The Vero cell culture multiplies actively on the microcarrier surface giving more than a 20-fold increase in 8 days. Mumps virus showed a high reproductive capacity in Vero cell culture and in primary JQE cells. Mumps virus-infected Vero cells produce 5-6 pools of virus-containing material with a mean infectious titre 8.2-8.3 lg HAE50/ml. The primary JQE culture infected with mumps virus can yield 2-3 pools of virus-containing material. The intensity of mumps virus replication in the latter directly depends on the multiplicity of infection. Hemadsorption test could be performed in mumps virus-infected cell cultures on microcarriers.
在连续传代的Vero细胞培养物以及在DEAE-葡聚糖A-50微载体上生长的日本鹌鹑胚胎（JQE）原代培养物中研究了腮腺炎病毒L-3疫苗株的繁殖情况。Vero细胞培养物在微载体表面上积极增殖，8天内增加了20倍以上。腮腺炎病毒在Vero细胞培养物和JQE原代细胞中显示出高繁殖能力。感染腮腺炎病毒的Vero细胞产生5-6批含病毒物质，平均感染滴度为8.2-8.3 lg HAE50/ml。感染腮腺炎病毒的JQE原代培养物可产生2-3批含病毒物质。腮腺炎病毒在后者中的复制强度直接取决于感染复数。血细胞吸附试验可在微载体上感染腮腺炎病毒的细胞培养物中进行。
