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[固定狂犬病病毒在悬浮生长的鹌鹑成纤维细胞培养物中的增殖]

[Reproduction of fixed rabies virus in a quail fibroblast culture growing in suspension].

作者信息

Nagieva F G, Bektemirova M S, Matevosian K Sh, Bektemirov T A, Pille E R

出版信息

Vopr Virusol. 1980 Jul-Aug(4):429-31.

PMID:7423971
Abstract

Reproduction of culture variant of fixed rabies virus was studied in cultures of quail fibroblasts growing in roller suspension cultures, as well as in monolayer cultures of Japanese quail embryos fixed on DEAE-Sephadex A-50 particles. Titers of the virus in cultures on the microcarrier were found to be similar with those in roller monolayer cultures, namely 5.75--6.5 lg LD50/ml. In shaker and roller suspension cultures the virus titers were lower: 4.5--5.25 lg LD50/ml.

摘要

在滚瓶悬浮培养的鹌鹑成纤维细胞培养物以及固定在DEAE-葡聚糖A-50颗粒上的日本鹌鹑胚胎单层培养物中,研究了固定狂犬病病毒培养变异株的繁殖情况。发现微载体培养物中的病毒滴度与滚瓶单层培养物中的相似,即5.75--6.5 lg LD50/ml。在摇床和滚瓶悬浮培养中,病毒滴度较低:4.5--5.25 lg LD50/ml。

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