脉冲和稳态标记以及放线菌素D追踪未感染的HeLa细胞中对5,6-二氯-1-β-D-呋喃核糖基苯并咪唑（DRB）具有抗性的核不均一RNA（hnRNA）。
Pulse and steady-state labelled and actinomycin D chased 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB)-resistant hnRNA from uninfected HeLa cells.
作者信息
Brysch B
出版信息
Acta Biochim Pol. 1983;30(3-4):311-24.
PMID:6201028
Abstract
The fraction of hnRNA synthesized in the presence of DRB in uninfected HeLa cells ranges in size from 4S to over 45S. High molecular weight DRB-resistant hnRNA has been demonstrated to decay after 2 h of actinomycin D chase. This fraction is composed of both poly(A)(+) and poly(A)(-) RNA molecules. The synthesis in the presence of DRB of short polyadenylated hnRNA was also observed. The nature of both hnRNA subfractions is discussed.
摘要
在未感染的HeLa细胞中，于DRB存在的情况下合成的核不均一RNA（hnRNA）片段大小范围从4S到超过45S。高分子量的DRB抗性hnRNA已被证明在放线菌素D追踪2小时后会降解。该片段由多聚腺苷酸（poly(A)）阳性和多聚腺苷酸阴性RNA分子组成。还观察到了在DRB存在的情况下短的多聚腺苷酸化hnRNA的合成。对这两种hnRNA亚组分的性质进行了讨论。
相似文献
1
2
Short capped hnRNA precursor chains in HeLa cells: continued synthesis in the presence of 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole.
Biochemistry. 1980 Jun 10;19(12):2743-8. doi: 10.1021/bi00553a032.
3
Recovery of HeLa cell population growth after treatment with 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB).
J Cell Physiol. 1983 Jul;116(1):26-34. doi: 10.1002/jcp.1041160106.
4
Early termination of heterogeneous nuclear RNA transcripts in mammalian cells: accentuation by 5,6-dichloro 1-beta-D-ribofuranosylbenzimidazole.
Proc Natl Acad Sci U S A. 1979 Nov;76(11):5750-4. doi: 10.1073/pnas.76.11.5750.
5
The inhibition by DRB (5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole) of hnRNA and mRNA production in HeLa cells.
Cell. 1976 Nov;9(3):473-80. doi: 10.1016/0092-8674(76)90092-1.
6
HeLa cell RNA and protein syntheses. Effects of long-term treatment with 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB).
Biochem Pharmacol. 1984 Feb 15;33(4):551-7. doi: 10.1016/0006-2952(84)90306-x.
7
5,6-Dichloro-1-Beta-D-ribofuranosylbenzimidazole inhibits initiation of nuclear heterogeneous RNA chains in HeLa cells.
Science. 1976 Oct 22;194(4263):431-3. doi: 10.1126/science.982026.
8
Posttranslational phosphorylation of specific chromosomal proteins and transcription of hnRNA genes in isolated nuclei: retention of in vivo sensitivity to 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB).
J Cell Biochem. 1985;29(2):115-26. doi: 10.1002/jcb.240290207.
9
Specific inhibition of hnRNA synthesis by 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole. Requirement of a free 3'-hydroxyl group, but not 2'- or 5'-hydroxyls.
Biochim Biophys Acta. 1982 May 31;697(2):213-20. doi: 10.1016/0167-4781(82)90079-3.
10
A DRB (5,6 dichloro-beta-D-ribofuranosylbenzimidazole)-resistant adenovirus mRNA.
Nucleic Acids Res. 1979 Nov 24;7(6):1405-18. doi: 10.1093/nar/7.6.1405.
Zotero 插件