Sehgal P B, Darnell J E, Tamm I
Cell. 1976 Nov;9(3):473-80. doi: 10.1016/0092-8674(76)90092-1.
5, 6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB; 75 muM) inhibited only 60-75% of nuclear heterogeneous RNA (hnRNA) synthesis in HeLa cells, while the appearance of labeled cytoplasmic poly(A)-containing messenger RNA (mRNA) was reduced by approximately 95%. DRB-sensitive and resistant transcripts spanned the entire size range of hnRNA molecules. The relative poly(A) content of DRB-resistant hnRNA molecules was the same as that of normal hnRNA. In contrast to the effects of 3'deoxyadenosine, DRB appeared to inhibit the initiation of hnRNA chains, but did not directly interfere with labeling of poly(A). We present an interpretation of these results and propose how the results might be used to gain more information about nuclear RNA metabolism.
5,6-二氯-1-β-D-呋喃核糖基苯并咪唑（DRB；75μM）仅抑制HeLa细胞中60%-75%的核不均一RNA（hnRNA）合成，而标记的细胞质含多聚腺苷酸信使RNA（mRNA）的出现减少了约95%。对DRB敏感和耐药的转录本涵盖了hnRNA分子的整个大小范围。对DRB耐药的hnRNA分子的相对多聚腺苷酸含量与正常hnRNA相同。与3'-脱氧腺苷的作用相反，DRB似乎抑制hnRNA链的起始，但不直接干扰多聚腺苷酸的标记。我们对这些结果进行了解释，并提出如何利用这些结果获取更多关于核RNA代谢的信息。
