Lagergård T, Branefors P
Acta Pathol Microbiol Immunol Scand C. 1983 Dec;91(6):371-6.
Cross-reactivity between capsular antigens of Haemophilus influenzae types a and b, and Streptococcus pneumoniae types 6A and 6B was studied by means of comparative double diffusion, quantitative precipitin tests and enzyme-linked immunosorbent assay (ELISA). Antisera against whole bacteria were employed. When capsular antigens of S. pneumoniae types 6A and 6B were compared by means of the double diffusion technique, three antigenic determinants were distinguished, two of them being common to the two serotypes. When pneumococcal capsular antigens were compared with H. influenzae capsular antigens, cross-reactive antibodies were detectable only in anti-pneumococcal sera. The heterologous pneumococcal polysaccharide preparation precipitated about 80% of the capsular antibodies in pneumococcal antisera, while heterologous H. influenzae polysaccharide precipitated only about 20-30% of the capsular antibodies in anti-H. influenzae sera. S. pneumoniae polysaccharide precipitated 10-20% of the antibodies in antisera against. H. influenzae, and H. influenzae polysaccharide precipitated about 20-25% of the antibodies in antisera against S. pneumoniae type 6A. Approximately the same degree of cross-reactivity was revealed when ELISA was employed. The absorption study of the antisera with D-ribitol-5-phosphate indicates that this structure is responsible for the cross-reactivity between S. pneumoniae and H. influenzae.
通过比较双向扩散、定量沉淀试验和酶联免疫吸附测定（ELISA）研究了a型和b型流感嗜血杆菌与6A和6B型肺炎链球菌荚膜抗原之间的交叉反应性。使用了抗全菌血清。当采用双向扩散技术比较6A和6B型肺炎链球菌的荚膜抗原时，可区分出三个抗原决定簇，其中两个是这两种血清型共有的。当将肺炎链球菌荚膜抗原与流感嗜血杆菌荚膜抗原进行比较时，仅在抗肺炎链球菌血清中可检测到交叉反应性抗体。异源肺炎链球菌多糖制剂沉淀了肺炎链球菌抗血清中约80%的荚膜抗体，而异源流感嗜血杆菌多糖仅沉淀了抗流感嗜血杆菌血清中约20%-30%的荚膜抗体。肺炎链球菌多糖沉淀了抗流感嗜血杆菌血清中10%-20%的抗体，流感嗜血杆菌多糖沉淀了抗6A 型肺炎链球菌血清中约20%-至25%的抗体。采用ELISA时显示出大致相同程度的交叉反应性。用D-核糖醇-5-磷酸对抗血清进行吸收研究表明，这种结构是肺炎链球菌和流感嗜血杆菌之间交叉反应性的原因。
