Symington J, Gurney T, Eliceiri G L
Biochem Biophys Res Commun. 1984 Apr 16;120(1):81-7. doi: 10.1016/0006-291x(84)91416-5.
Whole nuclear and cytoplasmic fractions from HeLa cells were analyzed in protein gel blots probed with either monoclonal anti-Sm or polyclonal anti-(U1)RNP antibodies. The cells were fractionated by a nonaqueous procedure, to minimize proteolysis and artifactual leakage of nuclear components to the cytoplasmic fraction. Unexpectedly, more reactive proteins were detected in the nucleus than shown earlier in partially purified small nuclear ribonucleoprotein particles (snRNPs). In addition, reactive polypeptides were now found in the cytoplasm. These results are discussed in reference to the possibility that the nucleus and cytoplasm of adult somatic human cells may have a more complex than anticipated set of populations of polypeptides bearing Sm or RNP antigenic determinants, including some proteins that might not be in snRNP form.
使用单克隆抗Sm抗体或多克隆抗（U1）RNP抗体对HeLa细胞的全核和细胞质组分进行蛋白质凝胶印迹分析。细胞通过非水程序进行分级分离，以尽量减少蛋白水解以及核成分向细胞质组分的人为泄漏。出乎意料的是，在细胞核中检测到的反应性蛋白比之前在部分纯化的小核核糖核蛋白颗粒（snRNP）中显示的更多。此外，现在在细胞质中也发现了反应性多肽。结合成年人体细胞的细胞核和细胞质可能具有比预期更复杂的带有Sm或RNP抗原决定簇的多肽群体这一可能性，对这些结果进行了讨论，其中包括一些可能不是snRNP形式的蛋白质。
