Martí A, Matsuda M, Sihag R K, Eliceiri G L
Biochem Biophys Res Commun. 1984 Aug 30;123(1):345-51. doi: 10.1016/0006-291x(84)90419-4.
We have examined the polypeptide pattern of small nuclear ribonucleoprotein particles that react with monoclonal anti-Sm antibodies or polyclonal anti-(U1)RNP antibodies. The fresh nuclear extracts analyzed were prepared from human cells that had been pulse-chased with a mixture of 15 3H-labeled amino acids. In contrast to previous reports in the literature, the apparent molecular weights of the major polypeptides that remained in the 1 M NaCl-washed ribonucleoprotein-antibody complexes were approximately 80000, 55000, 28000, 25000, 14000 and 9000, when probed with monoclonal anti-Sm antibodies, and about 69000, 58000 and 35000, when polyclonal anti-(U1)RNP antibodies were used.
我们检测了与单克隆抗Sm抗体或多克隆抗（U1）RNP抗体发生反应的小核核糖核蛋白颗粒的多肽模式。所分析的新鲜核提取物是从用15种3H标记氨基酸混合物进行脉冲追踪的人细胞中制备的。与文献中先前的报道相反，当用单克隆抗Sm抗体检测时，保留在1M NaCl洗涤的核糖核蛋白-抗体复合物中的主要多肽的表观分子量约为80000、55000、28000、25000、14000和9000；而当使用多克隆抗（U1）RNP抗体时，其表观分子量约为69000、58000和35000。
