Van der Linden P W, Deelder A M
Exp Parasitol. 1984 Apr;57(2):125-31. doi: 10.1016/0014-4894(84)90071-7.
The following fluorochromes were studied as probes for discrimination between living and dead Schistosoma mansoni schistosomula: ethidium bromide (EB), propidium iodide (PI), diamidinophenylindole (DAPI), and carboxyfluoresceine diacetate (C-FDA). While schistosomula stained with EB, PI, or C-FDA showed leakage of fluorochrome into the medium, this was not the case with DAPI. Dead schistosomula, which were stained with DAPI, showed an intense blue fluorescence, while living schistosomula were not stained even after prolonged incubation. In addition, the low DAPI concentration (1 microgram/ml) in the medium proved not to be toxic to the schistosomula, nor did it cause any background fluorescence. These properties make DAPI an ideal probe: the viability of S. mansoni schistosomula in cytotoxicity tests can be continuously monitored in tissue culture trays, using an inverted microscope with simultaneous transmitted light and incident fluorescent light illumination.
溴化乙锭（EB）、碘化丙啶（PI）、二脒基苯基吲哚（DAPI）和羧基荧光素二乙酸酯（C-FDA）。用EB、PI或C-FDA染色的童虫会出现荧光染料泄漏到培养基中的情况，但DAPI染色的童虫并非如此。用DAPI染色的死童虫呈现强烈的蓝色荧光，而活童虫即使长时间孵育后也未被染色。此外，培养基中低浓度的DAPI（1微克/毫升）被证明对童虫无毒，也不会引起任何背景荧光。这些特性使DAPI成为理想的探针：在细胞毒性试验中，使用配备透射光和入射荧光同时照明的倒置显微镜，可在组织培养皿中持续监测曼氏血吸虫童虫的活力。
