Gold D
Department of Human Microbiology, Sackler School of Medicine, Tel Aviv University, Israel.
Parasitol Res. 1997;83(2):163-9. doi: 10.1007/s004360050227.
Of various vital dyes used to assess schistosomula viability, toluidine blue enabled differential counting of the schistosomula on microscope slides but not in culture wells, whereas methylene blue could be added directly to the schistosomula suspension in culture wells of microtiter plates. Toluidine blue uptake by dead parasites was very fast. It mostly also partially stained damaged but not dead organisms. Its main disadvantage was rapid, nonspecific staining of live schistosomula, requiring prompt counting of a preparation and additional reliance on motility for assessment of viability. Methylene blue staining of dead worms was slower, but it did not stain the live worms until about 1 h after dye application, enabling its addition to a series of preparations for consecutive counting. It did not always stain flattened, dead schistosomula or it stained them an uncontrasting pale blue. This dye remarkably induced movement in seemingly inert and probably damaged worms, thus enabling determination of viability even following poor staining or a lack of staining.
在用于评估血吸虫幼虫活力的各种活性染料中，甲苯胺蓝能够在显微镜载玻片上对血吸虫幼虫进行鉴别计数，但在培养孔中不行，而亚甲蓝可以直接添加到微量滴定板培养孔中的血吸虫幼虫悬液中。死寄生虫对甲苯胺蓝的摄取非常快。它大多也会部分染色受损但未死亡的生物体。其主要缺点是对活的血吸虫幼虫进行快速、非特异性染色，需要对制剂进行快速计数，并额外依赖活动能力来评估活力。死虫的亚甲蓝染色较慢，但在染色后约1小时才会对活虫进行染色，这使得它可以添加到一系列制剂中进行连续计数。它并不总是能对扁平的死血吸虫幼虫进行染色，或者将它们染成对比度不高的浅蓝色。这种染料能显著诱导看似无活性且可能受损的虫体运动，从而即使在染色不佳或未染色的情况下也能确定其活力。
