Breuning M H, Spits H, de Vries J E, Iványi P
Hum Immunol. 1984 Apr;9(4):231-42. doi: 10.1016/0198-8859(84)90028-4.
The lymphoblastoid cell-line JY (HLA-A2,2;B7,7;C-; DR4,w6) was used to stimulate T cells from donor HG (HLA-A2,w23; B40,w44; Cw4; DRw6,7). Cloned CTL line were obtained by limiting dilution after tertiary stimulation. Strong cytotoxic activity on stimulator cells was found with all CTL clones obtained. One of the clones (HG-31 recognized a subtype of the HLA-B7 antigen. In this paper, we describe another long-term cloned CTL line (HG-61). This line, when tested on a panel of 107 target cells from unrelated individuals, recognized a subtype of HLA-B27 (B27 "K"). There was no significant association with any other HLA antigen. The cloned CTLs were T8+ and their cytotoxic activity could be blocked by the monoclonal antibody W6/32 which recognizes a framework determinant on HLA-A, -B, and -C molecules. In families, reactivity with cells of the CTL line (HG-61) segregated with HLA. It is concluded that the CTL line interacts with an antigenic determinant shared between the HLA-B7 antigen and JY and the subtype of HLA-B27 (B27 "K"), or detects products of a gene closely linked to HLA-B, not revealed by present-day serology.
淋巴母细胞系JY（HLA - A2,2；B7,7；C -；DR4,w6）用于刺激供体HG（HLA - A2,w23；B40,w44；Cw4；DRw6,7）的T细胞。经三次刺激后通过有限稀释获得克隆的CTL系。所获得的所有CTL克隆对刺激细胞均具有强烈的细胞毒活性。其中一个克隆（HG - 31）识别HLA - B7抗原的一个亚型。在本文中，我们描述了另一个长期克隆的CTL系（HG - 61）。当用来自无关个体的107个靶细胞组成的细胞板对该系进行检测时，它识别HLA - B27（B27“K”）的一个亚型。与任何其他HLA抗原均无显著关联。克隆的CTL为T8 +，其细胞毒活性可被识别HLA - A、 - B和 - C分子上一个框架决定簇的单克隆抗体W6/32阻断。在家族中，CTL系（HG - 61）与细胞的反应性与HLA共分离。得出的结论是，CTL系与HLA - B7抗原和JY之间共有的一个抗原决定簇以及HLA - B27（B27“K”）的亚型相互作用，或者检测到一个与HLA - B紧密连锁的基因产物，而这是当今血清学未揭示的。
