D-甘露醇向D-核糖的转化：大肠杆菌中一条新发现的途径。
Conversion of D-mannitol to D-ribose: a newly discovered pathway in Escherichia coli.
作者信息
Rosenberg H, Hardy C M
出版信息
J Bacteriol. 1984 Apr;158(1):69-72. doi: 10.1128/jb.158.1.69-72.1984.
A mutant (mtlD) strain of Escherichia coli unable to oxidize mannitol-1-phosphate to fructose-6-phosphate was used to study the fate of mannitol-1-phosphate. D-[1-14C]mannitol entered the cells via the phosphotransferase system and was phosphorylated equally at carbon 1 or 6. The label disappeared gradually from the mannitol-1-phosphate pool, and some 60% of the 14C was recovered in nucleic acids. Ribose was isolated from the purified RNA. The 14C label distribution in the isolated ribose precluded a simple hexose-to-pentose conversion by elimination of one terminal carbon from mannitol-1-phosphate. The 14C from mannitol-1-phosphate that did not enter macromolecules was found in CO2 and in some organic, non-phosphorylated compounds that were not identified. We suggest that the de novo synthesis of mannitol-1-phosphate in E. coli may be a reaction specifically dedicated to the biosynthesis of ribose.
利用一株无法将1-磷酸甘露醇氧化为6-磷酸果糖的大肠杆菌突变体（mtlD）来研究1-磷酸甘露醇的去向。D-[1-¹⁴C]甘露醇通过磷酸转移酶系统进入细胞，并在碳1或碳6处等量磷酸化。标记物逐渐从1-磷酸甘露醇池中消失，约60%的¹⁴C在核酸中被回收。从纯化的RNA中分离出核糖。分离出的核糖中¹⁴C标记分布排除了通过从1-磷酸甘露醇中去除一个末端碳进行简单的己糖到戊糖转化的可能性。未进入大分子的1-磷酸甘露醇中的¹⁴C存在于二氧化碳和一些未鉴定的有机非磷酸化化合物中。我们认为大肠杆菌中1-磷酸甘露醇的从头合成可能是一个专门用于核糖生物合成的反应。
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