大肠杆菌转酮醇酶突变体中的糖代谢
Sugar metabolism in transketolase mutants of Escherichia coli.
作者信息
Josephson B L, Fraenkel D G
出版信息
J Bacteriol. 1974 Jun;118(3):1082-9. doi: 10.1128/jb.118.3.1082-1089.1974.
Abstract
This paper continues the description of transketolase mutants of Escherichia coli; they are absolutely unable to grow on pentoses, but slightly "leaky" with respect to their aromatic requirement (B. L. Josephson and D. G. Fraenkel, 1969). Several experiments have explored the degree of leakiness and shown it to be low. There is little conversion of radioactive xylose to carbon dioxide. The labeling of ribose in cells grown on [1-(14)C]glucose and [2-(14)C]glucose accords with its origin being chiefly by the oxidative pathway. A mutant lacking both transketolase and gluconate-6-phosphate dehydrogenase has been constructed; it requires supplementation with pentose. Pentoses are inhibitory to growth of transketolase mutants, but high levels of pentose phosphates do not accumulate in this situation. Several experimental results are suggestive of regulation of metabolic flow in the oxidative branch of the hexose monophosphate shunt.
摘要
本文继续描述大肠杆菌转酮醇酶突变体；它们绝对不能在戊糖上生长，但在芳香族需求方面略有“渗漏”（B. L. 约瑟夫森和D. G. 弗伦克尔，1969年）。多项实验探究了渗漏程度，结果表明其程度较低。放射性木糖转化为二氧化碳的情况很少。在[1-(14)C]葡萄糖和[2-(14)C]葡萄糖上生长的细胞中核糖的标记情况与其主要通过氧化途径产生的来源一致。构建了一种同时缺乏转酮醇酶和6-磷酸葡萄糖酸脱氢酶的突变体；它需要补充戊糖。戊糖对转酮醇酶突变体的生长有抑制作用，但在这种情况下不会积累高水平的戊糖磷酸。几个实验结果表明磷酸戊糖途径氧化分支中代谢流存在调控。
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