Ewert D L, Munchus M S, Chen C L, Cooper M D
J Immunol. 1984 May;132(5):2524-30.
In this report, we describe the analysis of Ia-like antigens in the chicken by using a monoclonal antibody (CIa-1) reactive with monomorphic determinants of the Ia-like (B-L) antigens. This antibody reacts with determinants on B cells in all avian species tested, but does not detect antigens on lymphocytes of representative mammals, reptiles, and amphibians. In addition to B cells, this antibody defines a subpopulation of the monocyte-macrophage series and reacts with mitogen-activated T cells. Immunochemical analysis indicates that the CIa-1 reactive antigen is a 65,000-dalton glycoprotein consisting of an alpha-chain of 32,000 daltons noncovalently bound to a beta-chain of 27,000 daltons. Under nonreducing conditions, the beta-chain migrates with slightly faster mobility. Two-dimensional gel analysis indicates that the beta-chain is the more heterogeneous of the two chains. Thus, the antigen detected by CIa-1 antibody is similar in cell distribution and structure to the murine Ia antigens and human DR antigens. During in ovo development, Ia+Ig- cells were not found in the yolk sac but were detected in the spleen, mesonephros, and bursa of 9-day embryos. Two populations of Ia+Ig- cells were identified in the bursa: 40 to 60% of the bursacytes, mostly larger cells, exhibited brighter immunofluorescence reactivity than the smaller bursacytes.
在本报告中，我们描述了利用一种与类Ia（B-L）抗原的单态决定簇反应的单克隆抗体（CIa-1）对鸡的类Ia抗原进行的分析。该抗体与所有测试鸟类物种的B细胞上的决定簇发生反应，但在代表性哺乳动物、爬行动物和两栖动物的淋巴细胞上未检测到抗原。除B细胞外，该抗体还定义了单核细胞-巨噬细胞系列的一个亚群，并与丝裂原激活的T细胞发生反应。免疫化学分析表明，CIa-1反应性抗原是一种65,000道尔顿的糖蛋白，由一条32,000道尔顿的α链和一条27,000道尔顿的β链非共价结合而成。在非还原条件下，β链迁移速度稍快。二维凝胶分析表明，β链是两条链中更具异质性的。因此，CIa-1抗体检测到的抗原在细胞分布和结构上与小鼠Ia抗原和人类DR抗原相似。在胚胎发育过程中，卵黄囊中未发现Ia+Ig-细胞，但在9日龄胚胎的脾脏、中肾和法氏囊中检测到。在法氏囊中鉴定出两类Ia+Ig-细胞：40%至60%的法氏囊细胞，大多为较大细胞，比小法氏囊细胞表现出更强的免疫荧光反应性。
