Lotzová E, Savary C A, Khan A, Stringfellow D A
J Immunol. 1984 May;132(5):2566-70.
We tested the effect of 2-amino-5-bromo-6 phenyl-4 pyrimidinol (ABPP), an interferon (IFN) inducer, on NK cell cytotoxicity in various tissues of athymic and euthymic rats. Significant augmentation of NK cell cytotoxicity was observed in SP, PB, PE, lung, and liver of adult rats. Cytotoxic potential was also induced by ABPP in young rats, not exhibiting cytotoxicity before the treatment. Cytotoxic cells were detected in nylon wool-filtered fraction, and were not removed by carbonyl iron treatment. Separation on Percoll gradient indicated that ABPP-stimulated cytotoxic SPC resided in LGL-enriched fractions and some of them exhibited less dense characteristics than SPC from untreated rats. The ABPP-mediated cytotoxicity was thymus-independent; both athymic and euthymic rats were equally augmented. The observation that only tissues displaying NK cell augmentation produced significant levels of IFN suggest possible involvement of IFN in ABPP-mediated augmentation.
我们测试了干扰素（IFN）诱导剂2-氨基-5-溴-6-苯基-4-嘧啶醇（ABPP）对无胸腺和有胸腺大鼠各种组织中自然杀伤（NK）细胞细胞毒性的影响。在成年大鼠的脾脏、外周血、腹膜渗出液、肺和肝脏中观察到NK细胞细胞毒性显著增强。ABPP也诱导了幼鼠的细胞毒性潜能，这些幼鼠在治疗前未表现出细胞毒性。在尼龙毛过滤组分中检测到细胞毒性细胞，且羰基铁处理不能去除这些细胞。在Percoll梯度上进行分离表明，ABPP刺激的细胞毒性脾细胞存在于富含大颗粒淋巴细胞（LGL）的组分中，其中一些细胞表现出比未处理大鼠的脾细胞密度更低的特征。ABPP介导的细胞毒性不依赖胸腺；无胸腺和有胸腺大鼠的细胞毒性均同样增强。仅显示NK细胞增强的组织产生显著水平的IFN这一观察结果表明，IFN可能参与了ABPP介导的增强作用。
