Lotzová E, Savary C A, Gray K N, Raulston G L, Jardine J H
Exp Hematol. 1984 Sep;12(8):633-40.
We have demonstrated that Rowett and NIH strains of random-bred athymic and euthymic rats exhibited significant levels of natural killer (NK) cell cytotoxicity against various murine and human tumor cell lines. Rats of both strains displayed high NK cell cytotoxicity in peritoneal exudate, peripheral blood and spleen, and lower activities in lung and liver. No activity was detected in bone marrow. In general, athymic rats manifested higher levels of NK cell cytotoxicity and higher tumor-binding potential than euthymic animals. NK cell cytotoxicity was subject to late maturation and did not decline with age. Cytotoxic cells were not removed by carbonyl iron treatment and resided in both adherent and nonadherent fraction after nylon-wool filtration, with the majority of the cytotoxicity in the latter fraction. Percoll gradient separation studies indicated that, in general, the cytotoxic cells were of large granular lymphocyte morphology and of low density. Evaluation of tumor-binding capacity and cytotoxic profile of large granular lymphocytes has shown that the majority of these cells exhibited cytotoxic potential, but only a minority of them displayed tumor-binding capacity.
我们已经证明，罗维特（Rowett）和美国国立卫生研究院（NIH）品系的随机繁殖无胸腺和有胸腺大鼠，对各种鼠类和人类肿瘤细胞系表现出显著水平的自然杀伤（NK）细胞细胞毒性。两个品系的大鼠在腹腔渗出液、外周血和脾脏中均表现出较高的NK细胞细胞毒性，而在肺和肝脏中的活性较低。在骨髓中未检测到活性。一般来说，无胸腺大鼠比有胸腺动物表现出更高水平的NK细胞细胞毒性和更高的肿瘤结合潜力。NK细胞细胞毒性成熟较晚，且不随年龄下降。细胞毒性细胞不会被羰基铁处理去除，在尼龙毛过滤后存在于贴壁和非贴壁部分，大部分细胞毒性存在于后者部分。Percoll梯度分离研究表明，一般来说，细胞毒性细胞具有大颗粒淋巴细胞形态且密度较低。对大颗粒淋巴细胞的肿瘤结合能力和细胞毒性谱的评估表明，这些细胞中的大多数表现出细胞毒性潜力，但只有少数表现出肿瘤结合能力。
