Friedman S A, Bernstein I L, Enrione M, Marcus Z H
JAMA. 1984 May 25;251(20):2684-7.
Anaphylactic sensitivity to her husband's seminal fluid occurred in a 46-year-old woman six weeks after a hysterectomy. Her husband was azoospermic as a result of an elective vasectomy. A pooled sample of his seminal fluid was used for skin and laboratory tests. Fractionating (Sephadex G100) chromatography was also performed on his seminal fluid. The patient showed positive intracutaneous skin tests to his unfractionated seminal plasma and a Sephadex G100 fraction designated No. 3. This fraction was also passively transferable to a nonallergic volunteer and it stimulated histamine release from the patient's leukocytes. The dose-response effects of this fraction compared with unfractionated seminal plasma in these assays showed greater bioactivity. After informed consent was obtained, the patient was immunized to fraction 3 in a classic desensitization protocol. Two weeks after the final dose of immunotherapy, intercourse was performed without condoms in a medical facility and under the supervision of a physician. No adverse symptoms were experienced by the patient on this occasion. Regular and frequent sexual activity is required to maintain her tolerant state.
一名46岁女性在子宫切除术后六周出现对其丈夫精液的过敏反应。她的丈夫因选择性输精管结扎而无精子。采集其精液混合样本用于皮肤和实验室检测。还对其精液进行了分级分离（葡聚糖G100）色谱分析。患者对其未分级的精浆和标记为3号的葡聚糖G100级分进行皮内皮肤试验呈阳性。该级分也可被动转移至一名非过敏志愿者体内，并刺激患者白细胞释放组胺。在这些检测中，该级分与未分级精浆相比的剂量反应效应显示出更大的生物活性。在获得知情同意后，按照经典脱敏方案对患者进行了3号级分免疫治疗。在最后一剂免疫治疗两周后，在医疗机构且在医生监督下进行了无避孕套性交。患者此次未出现不良症状。需要规律且频繁的性活动来维持她的耐受状态。
