[血清胰弹性蛋白酶测定在大鼠实验性胰腺疾病中的价值]

[The value of estimation of serum pancreatic elastase in experimental pancreatic disorders in rats].

作者信息

Tei Y, Satake K, Umeyama K

出版信息

Nihon Geka Gakkai Zasshi. 1983 Dec;84(12):1279-85.

PMID:6201711

Abstract

The elastase levels in rat serum were measured in experimental pancreatic disorders by radioimmunoassay. After the induction of acute pancreatitis either by trypsin or normal saline, the serum elastase levels increased significantly. The serum elastase levels in trypsin induced pancreatitis were significantly higher than in normal saline induced pancreatitis. The levels in ascitic fluid were also higher in trypsin induced pancreatitis than in normal saline induced pancreatitis. Microscopically, haemorrhagic pancreatitis was found in trypsin induced pancreatitis and edemotous pancreatitis was found in normal saline induced pancreatitis. The serum elastase levels decreased significantly in rats who were treated by ethionine for long period. Microscopically, atrophic pancreas was found in those rats. These results suggest that the measurement of pancreatic elastase in either blood or ascitic fluid by radioimmunoassay is a useful diagnostic method for pancreatic disorders, especially in acute pancreatitis.

摘要

通过放射免疫分析法测定实验性胰腺疾病大鼠血清中的弹性蛋白酶水平。在用胰蛋白酶或生理盐水诱导急性胰腺炎后，血清弹性蛋白酶水平显著升高。胰蛋白酶诱导的胰腺炎中血清弹性蛋白酶水平显著高于生理盐水诱导的胰腺炎。胰蛋白酶诱导的胰腺炎腹水中的水平也高于生理盐水诱导的胰腺炎。显微镜下，胰蛋白酶诱导的胰腺炎中发现出血性胰腺炎，生理盐水诱导的胰腺炎中发现水肿性胰腺炎。长期用乙硫氨酸治疗的大鼠血清弹性蛋白酶水平显著降低。显微镜下，在这些大鼠中发现胰腺萎缩。这些结果表明，通过放射免疫分析法测定血液或腹水中的胰腺弹性蛋白酶是诊断胰腺疾病，尤其是急性胰腺炎的一种有用方法。