Baranov V I, Riabova L A, Spirin A S
Mol Biol (Mosk). 1984 Mar-Apr;18(2):350-7.
Using the solid-phase translation system technique where template poly(U) is covalently coupled to Sepharose through cleavable disulfide bridges translating monoribosomes carrying a polypeptide (polyPhe) of 10 to 20 amino acids long have been isolated. Both pre-translocation state and post-translocation state ribosomes have been obtained. It has been shown that the sedimentation coefficient of the pre-translocation state ribosomes exceeds that of the post-translocation state ribosomes by a magnitude of about 1S. This difference is independent on the sedimentation rate (hydrostatic pressure) in the range of 20 000 to 40 000 rev/min and, most likely, is not a direct contribution of the increase of the particle mass at the expense of an additional tRNA in the pre-translocation state ribosomes. Together with other data, this result suggests that translating ribosomes in the pre-translocation state are more compact than post-translocation state ribosomes.
利用固相翻译系统技术，其中模板聚（U）通过可裂解的二硫键与琼脂糖共价偶联，已分离出携带10至20个氨基酸长的多肽（多聚苯丙氨酸）的单核糖体。已获得转位前状态和转位后状态的核糖体。结果表明，转位前状态核糖体的沉降系数比转位后状态核糖体的沉降系数大约高1S。这种差异与20000至40000转/分钟范围内的沉降速率（静水压力）无关，而且很可能不是转位前状态核糖体中由于额外的tRNA导致颗粒质量增加的直接结果。与其他数据一起，这一结果表明，转位前状态的翻译核糖体比转位后状态的核糖体更紧凑。
