Spirin A S, Baranov V I, Polubesov G S, Serdyuk I N, May R P
J Mol Biol. 1987 Mar 5;194(1):119-26. doi: 10.1016/0022-2836(87)90720-0.
Translating ribosomes of Escherichia coli were prepared either in the pre-translocation or in the post-translocation states by a special technique based on the use of poly(U)-Sepharose columns where the template was coupled to the matrix through splittable -S-S- bridges. Elongation factors were absent from the final preparations. A neutron scattering study of the translating ribosomes in the two functional states was performed at different contrasts (various 1H2O/2H2O mixtures). Under conditions of a high contrast for the protein constituent the radius of gyration of the post-translocation-state ribosomes was found to be slightly greater than that of the pre-translocation-state ribosomes. Using the results of this study the conclusion can be drawn that translocation is accompanied by a spatial displacement of some parts of the ribosome with a magnitude of several ångström units.
通过一种基于使用聚（U）-琼脂糖柱的特殊技术，制备处于转位前或转位后状态的大肠杆菌翻译核糖体，其中模板通过可裂解的-S-S-桥与基质偶联。最终制剂中不存在延伸因子。在不同对比度（各种1H2O/2H2O混合物）下对处于两种功能状态的翻译核糖体进行了中子散射研究。在蛋白质成分具有高对比度的条件下，发现转位后状态核糖体的回转半径略大于转位前状态核糖体的回转半径。利用这项研究的结果可以得出结论，转位伴随着核糖体某些部分的空间位移，位移幅度为几个埃单位。
