Espinosa J, Botana L M, Fernández-Otero M P
Rev Esp Fisiol. 1983 Dec;39(4):441-5.
Mast cells from rat peritoneal fluid were studied under cholinergic stimulus. The cells were purified by a continuous Ficoll gradient and were challenged with acetylcholine and carbamylcholine. The preparations showed the typical dose-response pattern against compound 48/80 with a dose interval of 0.5-1 microM that clearly increased the secretion. However, the histamine release elicited by increasing concentrations of acetylcholine and carbamylcholine was of little magnitude and similar for all the concentrations; this kind of response has not a dose-response profile and does not appear to be explained by the presence of a cholinergic receptor on the mast cell cellular membranes. The cholinergic agents neither stimulated directly the mast cells nor did they cause any variation in the 48/80 response. The histamine release pattern obtained with these agents cannot support the hypothesis of a primary cholinergic receptor on mast cells. However, if such receptor existed, its action could only be of secondary importance, and in fact such action was not manifested with the compound 48/80.
对大鼠腹腔液中的肥大细胞在胆碱能刺激下进行了研究。细胞通过连续的Ficoll梯度进行纯化，并用乙酰胆碱和氨甲酰胆碱进行刺激。制剂显示出针对化合物48/80的典型剂量反应模式，剂量间隔为0.5 - 1 microM，明显增加了分泌。然而，由浓度不断增加的乙酰胆碱和氨甲酰胆碱引发的组胺释放量很小，且在所有浓度下都相似；这种反应没有剂量反应曲线，似乎也不能用肥大细胞膜上存在胆碱能受体来解释。胆碱能药物既不直接刺激肥大细胞，也不会使48/80反应发生任何变化。用这些药物获得的组胺释放模式不能支持肥大细胞上存在初级胆碱能受体的假说。然而，如果存在这样的受体，其作用只能是次要的，事实上，用化合物48/80并未表现出这种作用。
