[利用连续Ficoll梯度从大鼠腹腔液中分离肥大细胞]
[Isolation of mastocytes from rat peritoneal fluid using continuous Ficoll gradients].
作者信息
Botana L M, Espinosa J, Fernández-Otero M P
出版信息
Rev Esp Fisiol. 1983 Dec;39(4):435-40.
PMID:6201962
Abstract
A method for mast cells purification from rat peritoneal fluid is described. The method consists of a continuous Ficoll gradient between 20 and 22,5% (w/v) Ficoll and the cells are obtained with an 88% retrieval. Purity and viability were of 95% and 97% respectively. The cells so purified were functional against the compound 48/80 and the spontaneous secretion value was less than 8%.
摘要
描述了一种从大鼠腹腔液中纯化肥大细胞的方法。该方法包括在20%至22.5%（w/v）的菲可连续梯度，细胞回收率为88%。纯度和活力分别为95%和97%。如此纯化的细胞对化合物48/80有功能反应，自发分泌值小于8%。
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