Savard P, Blanchard L M, Mérand Y, Bédard P, Dussault J H, Dupont A
Brain Res. 1984 Mar;315(1):105-10. doi: 10.1016/0165-3806(84)90081-6.
Neonatal hypothyroidism was induced by injection of 131I on the first living day whilst neonatal hyperthyroidism was induced by daily administration of high doses of thyroxine (T4). Following decapitation, segments of the lumbar spinal cord were microdissected by a punch technique. We measured serotonin and 5-hydroxyindoleacetic acid (5-HIAA) contents by high performance liquid chromatography and both substance P and thyrotropin-releasing hormone (TRH) levels by radioimmunoassay. We demonstrated that: (1) neonatal hyperthyroidism decreased substance P and TRH levels in the dorsal and ventral horns respectively, without modifying serotonin and 5-HIAA contents; (2) neonatal hypothyroidism increased the concentration of substance P in dorsal horn, of TRH in ventral horn (confirming our previous work), of serotonin in ventral horn, and of 5-HIAA in both ventral and dorsal horns; (3) T4-replacement therapy abolishes hypothyroid effects on substance P, TRH, and 5-HIAA, but not on 5-HT accumulation; and (4) bovine growth hormone-replacement therapy has no therapeutic action on the hypothyroid-induced accumulation of substance P, TRH, serotonin and 5-HIAA.
在出生首日通过注射¹³¹I诱导新生大鼠甲状腺功能减退，而通过每日给予高剂量甲状腺素（T4）诱导新生大鼠甲状腺功能亢进。断头后，采用打孔技术对腰段脊髓节段进行显微解剖。我们通过高效液相色谱法测量血清素和5-羟吲哚乙酸（5-HIAA）含量，并通过放射免疫分析法测量P物质和促甲状腺激素释放激素（TRH）水平。我们证明：（1）新生大鼠甲状腺功能亢进分别降低了背角和腹角中P物质和TRH水平，而不改变血清素和5-HIAA含量；（2）新生大鼠甲状腺功能减退增加了背角中P物质的浓度、腹角中TRH的浓度（证实了我们之前的研究）、腹角中血清素的浓度以及腹角和背角中5-HIAA的浓度；（3）T4替代疗法消除了甲状腺功能减退对P物质、TRH和5-HIAA的影响，但对5-羟色胺（5-HT）积累没有影响；（4）牛生长激素替代疗法对甲状腺功能减退诱导的P物质、TRH、血清素和5-HIAA积累没有治疗作用。
