Savard P, Blanchard L M, Mérand Y, Dupont A
Brain Res. 1984 Aug;317(2):239-45. doi: 10.1016/0165-3806(84)90101-9.
In order to determine the role of growth hormone (GH) in the therapeutic effect of thyroxine (T4), we measured the content of serotonin (5-HT), 5-hydroxyindoleacetic acid (5-HIAA) and substance P in discrete brain nuclei of neonatally-induced hypothyroid rats and in neonatally-induced hypothyroid rats subsequently maintained on bovine growth hormone (b-GH) injections. Substance P was measured by radioimmunoassay whilst 5-HT and 5-HIAA levels were determined by HPLC with electrochemical detection. In neonatal hypothyroid rats, substance P concentration increased in 5 out of 11 brain nuclei dissected while 5-HT and 5-HIAA level increased in 7 out of 19 brain nuclei selected. Although b-GH-replacement therapy abolished the hypothyroid-induced accumulation of 5-HT and 5-HIAA in brain nuclei with exception of the substantia nigra zona reticulata, it did not influence the substance P accumulation. This suggests that the abnormal brain development observed during hypothyroidism may, in part, result from absence of growth hormone. We also observed that neonatal hyperthyroidism induced very little modification of 5-HT, 5-HIAA and substance P concentrations in discrete nuclei of the rat brain.
为了确定生长激素（GH）在甲状腺素（T4）治疗效果中的作用，我们测量了新生期诱导的甲状腺功能减退大鼠以及随后接受牛生长激素（b - GH）注射的新生期诱导的甲状腺功能减退大鼠离散脑核中5-羟色胺（5-HT）、5-羟吲哚乙酸（5-HIAA）和P物质的含量。P物质通过放射免疫测定法测量，而5-HT和5-HIAA水平通过高效液相色谱电化学检测法测定。在新生期甲状腺功能减退大鼠中，在解剖的11个脑核中的5个中P物质浓度增加，而在选择的19个脑核中的7个中5-HT和5-HIAA水平增加。尽管b - GH替代疗法消除了除黑质网状带外脑核中甲状腺功能减退诱导的5-HT和5-HIAA积累，但它并未影响P物质的积累。这表明在甲状腺功能减退期间观察到的脑发育异常可能部分是由于缺乏生长激素所致。我们还观察到新生期甲状腺功能亢进对大鼠脑离散核中5-HT、5-HIAA和P物质浓度的影响很小。
