Saksela O, Wahlström T, Meyer B, Vaheri A
Cancer Res. 1984 Jul;44(7):2942-6.
alpha-2-Macroglobulin (alpha 2M) was demonstrated in normal and mildly dysplastic cervical epithelium using immunohistochemical methods. In contrast, staining for alpha 2M diminished gradually with advancing epithelial dysplasia and was totally negative in truly neoplastic lesions. No in vitro synthesis could be detected in cultured ectocervical cells or in a corresponding malignant cell line (HeLa). However, alpha 2M was taken up from added human serum by cultured normal ectocervical cells, whereas HeLa cells remained negative also under these conditions. These and previous results suggest that normal cells may have the capacity to synthesize or endocytose alpha 2M, thus possibly regulating their pericellular proteolysis, while these functions may be defective in malignantly transformed cells.
采用免疫组化方法在正常及轻度发育异常的宫颈上皮中检测到α-2-巨球蛋白（α2M）。相比之下，随着上皮发育异常程度的加重，α2M染色逐渐减弱，在真正的肿瘤性病变中则完全呈阴性。在培养的宫颈外细胞或相应的恶性细胞系（HeLa）中未检测到体外合成。然而，培养的正常宫颈外细胞可从添加的人血清中摄取α2M，而在这些条件下HeLa细胞仍呈阴性。这些及之前的结果表明，正常细胞可能具有合成或内吞α2M的能力，从而可能调节其细胞周围的蛋白水解作用，而这些功能在恶性转化细胞中可能存在缺陷。
