Saksela O, Wahlström T, Lehtovirta P, Seppälä M, Vaheri A
Cancer Res. 1981 Jun;41(6):2507-13.
alpha 2-Macroglobulin (alpha 2M) was demonstrated in normal syncytiotrophoblasts of both early and full-term human placentas using immunocytological staining. alpha 2M was also detected in hydatidiform moles, the benign tumors of proliferating syncytiotrophoblasts. In contrast, no alpha 2M was detected in invasive moles or choriocarcinomas. In culture conditions, both normal syncytiotrophoblasts and choriocarcinoma cells, identified by production of human chorionic gonadotropin, were negative when stained for alpha 2M or when studied using metabolic labeling and immunoprecipitation or radioimmunoassay. However, alpha 2M was taken up from added human serum by the cultured syncytiotrophoblasts, whereas choriocarcinoma cells remained negative also under these conditions. The possible role of alpha 2M in the regulation of proteolysis in cell invasion is considered.
采用免疫细胞化学染色法在早期和足月人胎盘的正常合体滋养层细胞中证实了α2-巨球蛋白（α2M）的存在。在葡萄胎（增殖性合体滋养层细胞的良性肿瘤）中也检测到了α2M。相比之下，在侵蚀性葡萄胎或绒毛膜癌中未检测到α2M。在培养条件下，通过人绒毛膜促性腺激素的产生鉴定的正常合体滋养层细胞和绒毛膜癌细胞，在进行α2M染色时，或使用代谢标记、免疫沉淀或放射免疫测定法进行研究时均呈阴性。然而，培养的合体滋养层细胞从添加的人血清中摄取了α2M，而在这些条件下绒毛膜癌细胞仍呈阴性。本文考虑了α2M在细胞侵袭中蛋白水解调节方面的可能作用。
