Bassant M H, Cathala F, Court L, Gourmelon P, Hauw J J
Electroencephalogr Clin Neurophysiol. 1984 Jun;57(6):541-7. doi: 10.1016/0013-4694(84)90090-7.
Scrapie was transmitted (1st passage) to various strains of rats through intracerebral (IC) inoculation of sheep-scrapie, mouse-scrapie and hamster-scrapie brains. For the 2nd passage, Wistar rats were chosen and inoculated IC with rat-scrapie brain from the 1st passage. Three groups of 12 rats were considered (S for sheep, M for mouse, H for hamster). Electrodes were chronically implanted for EEG recording. From 100 to 110 days after inoculation, EEG abnormalities were observed, consisting of spindle-shaped bursts of diphasic spikes sporadically occurring during quiet wakefulness. Between 130 and 230 days, EEG signs diversified and became more pronounced. Simultaneously, changes occurred in the sleep-wakefulness cycle: active wakefulness decreased and was replaced by quiet wakefulness (rats were motionless , as if prostrate ). Slow wave sleep diminished, giving way to an unstable low voltage sleep. EEG disturbances clearly preceded the onset of the clinical signs (the latter appearing at about the 8th month). The disease was fairly similar in the S, M and H groups. The interest of the experimental scrapie rat model is discussed in the framework of current research on the neurophysiology of the subacute spongiform encephalopathies.
通过脑内（IC）接种羊瘙痒病、小鼠瘙痒病和仓鼠瘙痒病的脑组织，将瘙痒病（第1代）传播给了各种品系的大鼠。在第2代时，选择了Wistar大鼠，并脑内接种第1代大鼠瘙痒病的脑组织。实验分为三组，每组12只大鼠（S代表羊，M代表小鼠，H代表仓鼠）。长期植入电极以记录脑电图（EEG）。接种后100至110天，观察到脑电图异常，表现为在安静觉醒期间偶尔出现的纺锤形双相尖峰爆发。在130至230天之间，脑电图体征多样化且变得更加明显。同时，睡眠-觉醒周期发生了变化：主动觉醒减少，被安静觉醒所取代（大鼠一动不动，好像俯卧着）。慢波睡眠减少，被不稳定的低电压睡眠所取代。脑电图紊乱明显先于临床症状出现（后者大约在第8个月出现）。S、M和H组的疾病情况相当相似。在当前关于亚急性海绵状脑病神经生理学的研究框架内，讨论了实验性瘙痒病大鼠模型的意义。
