Lane E B, Goodman S L, Trejdosiewicz L K
EMBO J. 1982;1(11):1365-72. doi: 10.1002/j.1460-2075.1982.tb01324.x.
The behaviour of keratin filaments during cell division was examined in a wide range of epithelial lines from several species. Almost half of them show keratin disruption as described previously: by immunofluorescence, filaments are replaced during mitosis by a 'speckled' pattern of discrete cytoplasmic dots. In the electron microscope these ' speckles ' are seen as granules around the cell periphery, just below the actin cortical mesh, with no detectable 10 nm filament structure inside them and no keratin filament bundles in the rest of the cytoplasm. A time course of the filament reorganization was constructed from double immunofluorescence data; filaments are disrupted in prophase, and the filament network is intact again by cytokinesis. The phenomenon is restricted to cells rich in keratin filaments, such as keratinocytes; it is unrelated to the co-existence of vimentin in many of these cells, and vimentin is generally maintained as filaments while the keratin is restructured. Some resistance to the effect may be conferred by an extended cycle time. Filament reorganization takes place within minutes, so that a reversible mechanism seems more likely than one involving de novo protein synthesis, at this metabolically quiet stage of the cell cycle.
在来自多个物种的多种上皮细胞系中，研究了角蛋白丝在细胞分裂过程中的行为。其中近一半表现出如先前所述的角蛋白破坏：通过免疫荧光观察，丝在有丝分裂期间被离散的细胞质点状“斑点”模式所取代。在电子显微镜下，这些“斑点”表现为细胞周边、肌动蛋白皮质网下方的颗粒，内部没有可检测到的10纳米丝状结构，细胞质其余部分也没有角蛋白丝束。根据双重免疫荧光数据构建了丝重组的时间进程；丝在前期被破坏，到胞质分裂时丝网络再次完整。这种现象仅限于富含角蛋白丝的细胞，如角质形成细胞；它与许多这些细胞中波形蛋白的共存无关，并且在角蛋白重组时波形蛋白通常保持为丝状。延长的细胞周期时间可能赋予一些对这种效应的抗性。丝重组在几分钟内发生，因此在细胞周期这个代谢平静阶段，一种可逆机制似乎比涉及从头合成蛋白质的机制更有可能。
