Trejdosiewicz L K, Smolira M A, Hodges G M, Goodman S L, Livingston D C
J Microsc. 1981 Aug;123(Pt 2):227-36. doi: 10.1111/j.1365-2818.1981.tb01297.x.
Expression of cell surface fibronectin in cultures of untransformed fibroblasts is well documented, but little is known of its presence and distribution in cultured epithelial cells. Using species monospecific anti-fibronectin antibodies, the distribution of fibronectin in untransformed fibroblasts and in normal and neoplastic bladder epithelial cells was characterized by indirect labelling experiments using immunogold scanning electron microscopy (SEM). The surface matrix of fibronectin expressed in rodent and human fibroblast cell lines was demonstrated with ease by SEM of gold-tagged second antibodies. However, no fibronectin could be detected on any of the mouse and human bladder epithelium-derived cells studied in single or in mixed epithelial-fibroblast cultures. These SEM-immunogold observations were compared to and confirmed by immunofluorescence and immunoperoxidase microscopy. Immunofluorescence and SEM localization of the fibronectin in the extracellular matrix presented similar distribution patterns but the higher resolution of the SEM provided a more detailed analysis.
未转化的成纤维细胞培养物中细胞表面纤连蛋白的表达已有充分记载，但对于其在培养的上皮细胞中的存在和分布却知之甚少。使用种属特异性抗纤连蛋白抗体，通过免疫金扫描电子显微镜（SEM）间接标记实验，对未转化的成纤维细胞以及正常和肿瘤性膀胱上皮细胞中纤连蛋白的分布进行了表征。通过金标记二抗的SEM，很容易在啮齿动物和人类成纤维细胞系中表达的纤连蛋白表面基质中得到证实。然而，在单一或混合上皮 - 成纤维细胞培养物中研究的任何小鼠和人类膀胱上皮来源的细胞上均未检测到纤连蛋白。这些SEM免疫金观察结果与免疫荧光和免疫过氧化物酶显微镜检查结果进行了比较并得到证实。纤连蛋白在细胞外基质中的免疫荧光和SEM定位呈现出相似的分布模式，但SEM的更高分辨率提供了更详细的分析。
