Diamond A G, Larkins A P, Wright B, Ellis S T, Butcher G W, Howard J C
Eur J Immunol. 1984 May;14(5):405-12. doi: 10.1002/eji.1830140505.
Over 300 monoclonal IgG alloantibodies have been prepared against RT1Aa , the class I major histocompatibility complex molecule of the DA rat. In this study a combination of techniques is exploited to show that all these antibodies can be allocated to 9 antigenic sites which form a continuous antigenic surface, that is, no site is completely isolated from the rest. The results suggest that techniques for the identification of antigenic sites using competitive inhibition of monoclonal antibody binding are generally valid, in the sense that competition between antibodies appears most commonly to represent competition between combining sites for a structural feature of the antigenic surface. From the distribution of antibodies between sites, it is clear that the RT1Aa molecule has three immunogenic areas against which nearly all the antibodies studied were directed. Of these areas one is both antigenically complex, consisting of four closely spaced sites, and remarkably immunodominant. Antibodies directed at sites between the major areas are extremely rare.
已经制备了300多种针对DA大鼠I类主要组织相容性复合体分子RT1Aa的单克隆IgG同种抗体。在本研究中，采用了多种技术相结合的方法来表明，所有这些抗体都可以被分配到9个抗原位点，这些位点形成了一个连续的抗原表面，也就是说，没有一个位点与其他位点完全隔离。结果表明，使用单克隆抗体结合的竞争性抑制来鉴定抗原位点的技术通常是有效的，从这个意义上说，抗体之间的竞争最常见地表现为结合位点之间对抗原表面结构特征的竞争。从抗体在位点之间的分布情况来看，很明显RT1Aa分子有三个免疫原性区域，几乎所有研究的抗体都针对这些区域。在这些区域中，有一个区域在抗原上很复杂，由四个紧密间隔的位点组成，并且具有显著的免疫优势。针对主要区域之间位点的抗体极其罕见。
