在细胞质中合成的衣藻叶绿体核糖体蛋白是以前体形式存在的。
Chloroplast ribosomal proteins of Chlamydomonas synthesized in the cytoplasm are made as precursors.
作者信息
Schmidt R J, Myers A M, Gillham N W, Boynton J E
出版信息
J Cell Biol. 1984 Jun;98(6):2011-8. doi: 10.1083/jcb.98.6.2011.
Abstract
Polyadenylated RNA from Chlamydomonas was translated in a cell-free rabbit reticulocyte system that employed [35S]methionine. Antibodies made to four chloroplast ribosomal proteins synthesized in the cytoplasm and imported into the organelle were used for indirect immunoprecipitation of the labeled translation products, which were subsequently visualized on fluorographs of SDS gels. The cytoplasmically synthesized chloroplast ribosomal proteins were first seen as precursors with apparent molecular weights of 1,000 to 6,000 greater than their respective mature forms. Processing of the ribosomal protein precursors to mature proteins was affected by adding a postribosomal supernatant that had been extracted from cells of Chlamydomonas. In contrast to the chloroplast ribosomal proteins synthesized in the cytoplasm, two such proteins made within the chloroplast were found to be synthesized in mature form in cell-free wheat germ translation systems programmed with nonpolyadenylated RNA.
摘要
来自衣藻的多聚腺苷酸化RNA在使用[35S]甲硫氨酸的无细胞兔网织红细胞系统中进行翻译。针对在细胞质中合成并导入细胞器的四种叶绿体核糖体蛋白制备的抗体，用于对标记的翻译产物进行间接免疫沉淀，随后在SDS凝胶的荧光照片上观察这些产物。在细胞质中合成的叶绿体核糖体蛋白最初以比其各自成熟形式的表观分子量高1000至6000的前体形式出现。核糖体蛋白前体加工成成熟蛋白受到添加从衣藻细胞中提取的核糖体后上清液的影响。与在细胞质中合成的叶绿体核糖体蛋白不同，在用非多聚腺苷酸化RNA编程的无细胞小麦胚芽翻译系统中，发现叶绿体中产生的两种此类蛋白以成熟形式合成。
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