衣藻中叶绿体核糖体蛋白的合成位点。
Sites of synthesis of chloroplast ribosomal proteins in Chlamydomonas.
作者信息
Schmidt R J, Richardson C B, Gillham N W, Boynton J E
出版信息
J Cell Biol. 1983 May;96(5):1451-63. doi: 10.1083/jcb.96.5.1451.
Abstract
Cells of Chlamydomonas reinhardtii were pulse-labeled in vivo in the presence of inhibitors of cytoplasmic (anisomycin) or chloroplast (lincomycin) protein synthesis to ascertain the sites of synthesis of chloroplast ribosomal proteins. Fluorographs of the labeled proteins, resolved on two-dimensional (2-D) charge/SDS and one-dimensional (1-D) SDS-urea gradient gels, demonstrated that five to six of the large subunit proteins are products of chloroplast protein synthesis while 26 to 27 of the large subunit proteins are synthesized on cytoplasmic ribosomes. Similarly, 14 of 31 small subunit proteins are products of chloroplast protein synthesis, while the remainder are synthesized in the cytoplasm. The 20 ribosomal proteins shown to be made in the chloroplast of Chlamydomonas more than double the number of proteins known to be synthesized in the chloroplast of this alga.
摘要
莱茵衣藻细胞在存在细胞质（茴香霉素）或叶绿体（林可霉素）蛋白质合成抑制剂的情况下进行体内脉冲标记，以确定叶绿体核糖体蛋白的合成位点。在二维（2-D）电荷/SDS和一维（1-D）SDS-尿素梯度凝胶上分离的标记蛋白的荧光照片表明，大亚基蛋白中有五到六种是叶绿体蛋白质合成的产物，而大亚基蛋白中有26到27种是在细胞质核糖体上合成的。同样，31种小亚基蛋白中有14种是叶绿体蛋白质合成的产物，其余的在细胞质中合成。已证明在莱茵衣藻叶绿体中产生的20种核糖体蛋白比已知在这种藻类叶绿体中合成的蛋白数量增加了一倍多。
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