Combs G F, Liu C H, Lu Z H, Su Q
J Nutr. 1984 May;114(5):964-76. doi: 10.1093/jn/114.5.964.
Day-old chicks were fed a practical-type diet based on corn- and soybean meal produced in a severely Se-deficient area of northeastern China. The diet contained 0.007 ppm Se and resulted in marked decreases in the activities of Se-dependent glutathione peroxidase in plasma and pancreas within 6 days of feeding. Chicks fed this basal diet showed histological signs of acinar atrophy of the pancreas, hyaline body formation, vacuolation and cytoplasmic shrinkage by 18 days and significantly elevated activities of amylase in plasma by 30 days. Each of these changes was prevented by supplementing the basal diet with Se to bring it to a level of 0.20 ppm. Chicks fed a Se-deficient purified diet based on crystalline amino acids also showed decreased Se-dependent glutathione peroxidase activities in plasma and pancreas, pancreatic damage as evidenced by histological examination and increases in plasma amylase activities. However, these signs of nutritionally induced pancreatic atrophy occurred sooner and were of greater magnitude than those observed in Se-deficient chicks fed the practical diet within the 30-day experimental period. These results, therefore, constitute the first report of nutritionally induced pancreatic atrophy in Se-deficient chicks fed a diet containing intact protein, and we suggest that a factor(s) associated with the practical diet acts to partially protect the chicks from this pathological consequence of severe Se deficiency.
将1日龄雏鸡饲喂以中国东北严重缺硒地区生产的玉米和豆粕为基础的实用型日粮。该日粮含硒量为0.007 ppm，饲喂6天内血浆和胰腺中硒依赖性谷胱甘肽过氧化物酶的活性显著下降。饲喂这种基础日粮的雏鸡在18天时出现胰腺腺泡萎缩、透明体形成、空泡化和细胞质收缩的组织学迹象，30天时血浆淀粉酶活性显著升高。通过在基础日粮中添加硒使其达到0.20 ppm的水平，可防止上述每种变化。饲喂以结晶氨基酸为基础的缺硒纯化日粮的雏鸡，血浆和胰腺中硒依赖性谷胱甘肽过氧化物酶活性也降低，组织学检查显示胰腺受损，血浆淀粉酶活性增加。然而，在30天的实验期内，这些营养性诱导的胰腺萎缩迹象比饲喂实用日粮的缺硒雏鸡出现得更早，程度也更严重。因此，这些结果构成了关于饲喂含完整蛋白质日粮的缺硒雏鸡营养性诱导胰腺萎缩的首次报道，我们认为与实用日粮相关的一个或多个因素可部分保护雏鸡免受严重缺硒的这种病理后果。
