Whipple T L, Caspari R B, Meyers J F
Lasers Surg Med. 1984;3(4):297-304. doi: 10.1002/lsm.1900030406.
This study investigates the healing potential of the peripheral rim of rabbit meniscus following laser subtotal meniscectomy. Thirty animals were operated with 23 bilateral meniscectomies and 7 sham controls. When sacrificed at intervals up to 6 months, the harvested peripheral rims demonstrated a devitalized layer of fibrocartilage averaging 17 micron in thickness, indicating an extremely superficial thermal effect of the laser energy utilizing power densities of 20 to 38 W. Viable chondrocytes and minimal alteration of collagen fibril structure and orientation were noted within 20 to 50 micron of the meniscus free margin. By 2 weeks, a proliferation of vascular buds near the free margin of the meniscus was observed with an increase in ground substance production and chondrocyte population. By 10 weeks, the free margin was completely sealed and was composed of logically oriented collagen fibers and only slightly increased numbers of chondrocytes. It was concluded that the healing potential of rabbit meniscus is unimpaired following laser resection of the central margin, and that the repair process is mediated by a proliferation of chondrocytes, supported in part by neovascularity within the substance of the meniscus.
本研究调查了兔半月板激光次全切除术后外周缘的愈合潜力。对30只动物进行了手术，其中23例为双侧半月板切除术，7例为假手术对照。在长达6个月的不同时间点处死后，采集的外周缘显示出一层平均厚度为17微米的失活纤维软骨层，这表明利用20至38瓦功率密度的激光能量产生的热效应极为浅表。在半月板游离缘20至50微米范围内观察到存活的软骨细胞，且胶原纤维结构和取向仅有微小改变。到2周时，观察到半月板游离缘附近血管芽增殖，同时细胞外基质产生增加，软骨细胞数量增多。到10周时，游离缘完全封闭，由排列有序的胶原纤维组成，软骨细胞数量仅略有增加。研究得出结论，兔半月板中央缘激光切除术后其愈合潜力未受损害，修复过程由软骨细胞增殖介导，部分由半月板实质内的新生血管提供支持。
