Hsu E, Du Pasquier L
Mol Immunol. 1984 Apr;21(4):257-70. doi: 10.1016/0161-5890(84)90096-8.
Monoclonal antibodies raised against Xenopus Ig recognize antigenic determinants on IgM and low mol. wt Ig (LMW Ig or IgY ). On SDS-PAGE two forms of mu were distinguished in the supernatant and cell lysate of tunicamycin-treated spleen cell culture. The two bands of v observed in serum appear to be the result of carbohydrate heterogeneity. The light chains resolved into two distinct bands which differed in peptide maps; the more slowly migrating band of light chain was preferentially associated with v. Patterns of cross-reactivity between the immunoglobulins of 11 species of Xenopus and three subspecies of Xenopus laevis were obtained; the polymorphism of mu and v antigenic determinants and its implications for species divergence are discussed.
针对非洲爪蟾免疫球蛋白产生的单克隆抗体可识别免疫球蛋白M和低分子量免疫球蛋白（LMW Ig或IgY）上的抗原决定簇。在衣霉素处理的脾细胞培养上清液和细胞裂解物中，十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）鉴别出两种形式的μ链。血清中观察到的两条ν链带似乎是碳水化合物异质性的结果。轻链解析为两条不同的带，其肽图不同；迁移较慢的轻链带优先与ν链相关。获得了11种非洲爪蟾和非洲爪蟾三个亚种的免疫球蛋白之间的交叉反应模式；讨论了μ链和ν链抗原决定簇的多态性及其对物种分化的影响。
