Shioda T, Hidaka Y, Kanda T, Shibuta H, Nomoto A, Iwasaki K
Nucleic Acids Res. 1983 Nov 11;11(21):7317-30. doi: 10.1093/nar/11.21.7317.
The sequence of 3,687 nucleotides from the 3' end of the Sendai virus genome (Z strain) was determined by a molecular cloning technique followed by rapid sequence analysis. Two large open reading frames, one consisting of 1,572 nucleotides and the other of 1,704 nucleotides, were observed in the region, that is OP-1 and OP-2 from the 3' end of the genome. The amino acid sequences of the gene products were predicted from the observed sequence. Determination of amino acid compositions of viral proteins, P, HN, Fo, NP and M, led us to conclude that NP and P are the gene products of OP-1 and OP-2, respectively. An additional open reading frame consisting of 612 nucleotides (OP-3) was discovered in the 3' most proximal region of OP-2. The predicted product of OP-3 was considered to be viral non-structural protein C. The leader sequence of 51 nucleotides at the 3' terminal of the genome and consensus sequences at 3' and 5' ends of each gene for proteins NP and P were identified.
通过分子克隆技术及快速序列分析，确定了仙台病毒基因组（Z株）3'端3687个核苷酸的序列。在该区域观察到两个大的开放阅读框，一个由1572个核苷酸组成，另一个由1704个核苷酸组成，即从基因组3'端起的OP-1和OP-2。根据观察到的序列预测了基因产物的氨基酸序列。对病毒蛋白P、HN、Fo、NP和M的氨基酸组成的测定使我们得出结论，NP和P分别是OP-1和OP-2的基因产物。在OP-2的最靠近3'端的区域发现了一个由612个核苷酸组成的额外开放阅读框（OP-3）。OP-3的预测产物被认为是病毒非结构蛋白C。确定了基因组3'末端的51个核苷酸的前导序列以及NP和P蛋白每个基因3'和5'末端的共有序列。
